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The fiber-optic probe is an essential component of many quantitative fluorescence spectroscopy systems, enabling delivery of excitation light and collection of remitted fluorescence in a wide variety of microfluidic fluorescence detection system, clinical and laboratory situations. There has been an incentive in this field to push sample volumes and detection limits to lower levels. Detection of fluorescence from a low level sample is challenging for a fiber-optic probe, especially when an inexpensive and robust construction is desired therefore The effects of fiber parameters like fiber diameter, numerical aperture, core-clad ratio, arrangement of collection fibers around the excitation fiber and dead space between them, and optical properties of the medium on the performance of probes have been analyzed and compared with the results of previous observations.